Difference between revisions of "Part:BBa K1336007:Design"
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===Source=== | ===Source=== | ||
− | From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided | + | From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided by the Microbial & Enzyme Technology Lab Research Group, from Instituto de Tecnologia Química e Biológica in Lisbon University. |
===References=== | ===References=== |
Revision as of 17:53, 21 October 2014
LacI Expression Cassette with BsDyP gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1741
Illegal BsaI site found at 1750
Illegal BsaI site found at 2716
Illegal SapI site found at 2146
Illegal SapI site found at 2504
Illegal SapI.rc site found at 2660
Design Notes
N/A
Source
From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided by the Microbial & Enzyme Technology Lab Research Group, from Instituto de Tecnologia Química e Biológica in Lisbon University.