Difference between revisions of "Part:BBa K1321091:Design"
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===Source=== | ===Source=== | ||
| − | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the | + | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. |
===References=== | ===References=== | ||
Revision as of 17:51, 21 October 2014
Phytochelatin (PC) EC20 fused to CBDclos
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 133 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.