Difference between revisions of "Part:BBa K1336006"
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| − | An antisense fragment designed using the sense/coding strand of the ispB [https://parts.igem.org/Part:BBa_K1336005] gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of quinones within E. coli metabolic processes. At the 5' end, there is also a LacI expression cassette [https://parts.igem.org/Part:BBa_K314103] IPTG-inducible promoter to drive ispB expression. | + | An antisense fragment designed using the sense/coding strand of the ispB [https://parts.igem.org/Part:BBa_K1336005] gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of the side-chains of isoprene quinones within E. coli metabolic processes. At the 5' end, there is also a LacI expression cassette [https://parts.igem.org/Part:BBa_K314103] IPTG-inducible promoter to drive ispB expression. |
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Latest revision as of 16:36, 21 October 2014
LacI Expression Cassette with ispB RC gene
An antisense fragment designed using the sense/coding strand of the ispB [1] gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of the side-chains of isoprene quinones within E. coli metabolic processes. At the 5' end, there is also a LacI expression cassette [2] IPTG-inducible promoter to drive ispB expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]