Difference between revisions of "Part:BBa K1336005"

 
 
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<partinfo>BBa_K1336005 short</partinfo>
 
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An antisense fragment designed using the sense/coding strand of the ispB gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of quinones within E. coli metabolic processes.
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A fragment of the reverse complement strand of the ispB gene as an antisense RNA method for gene silencing of ispB, which codes for octanprenyl diphosphate synthase. This is a crucial enzyme in the <em>E. coli</em> metabolism, being responsible for the synthesis of the side chains of isoprene quinones.
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Latest revision as of 16:11, 21 October 2014

Antisense for octaprenyl diphosphate synthase (ispB gene)

A fragment of the reverse complement strand of the ispB gene as an antisense RNA method for gene silencing of ispB, which codes for octanprenyl diphosphate synthase. This is a crucial enzyme in the E. coli metabolism, being responsible for the synthesis of the side chains of isoprene quinones.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]