Difference between revisions of "Part:BBa K1456001:Design"

Revision as of 14:20, 21 October 2014

Human Tissue Plasminogen Activator (htPA)

Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.

The specified primers were put into colony PCR. As it can be seen in the results above, a right insert was not achieved. This process was repeated several times but no result was achieved. Seeing that ligation did not provide a solution to the problem, synthetically produced inserts were ordered.

Assembly Compatibility:

@@ Line 5: / Line 5: @@
 ===Cloning===
-[[File:ATOMS-tpa_results3.png|400px|]]
+<center>[[File:ATOMS-tpa_results3.png|400px|]]</center><br/>
 *Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.
+[[File:https://static.igem.org/mediawiki/2014/0/0c/ATOMS-tpa_results5.png]]
+*The specified primers were put into colony PCR. As it can be seen in the results above, a right insert was not achieved. This process was repeated several times but no result was achieved. Seeing that ligation did not provide a solution to the problem, synthetically produced inserts were ordered.
 <partinfo>BBa_K1456001 SequenceAndFeatures</partinfo>