Difference between revisions of "Part:BBa K1456001:Design"

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===Cloning===
 
===Cloning===
  
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<center>[[File:ATOMS-tpa_results3.png|400px|]]<center/>
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[[File:ATOMS-tpa_results3.png|400px|]]
  
 
*Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.
 
*Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.
  
 
<partinfo>BBa_K1456001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1456001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===

Revision as of 14:15, 21 October 2014

Human Tissue Plasminogen Activator (htPA)

ATOMS-tpa results1.png

Cloning

ATOMS-tpa results3.png

  • Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 958

Design Notes

None

Source

We clonned this part from human hepatocellular carcinoma cell genome cDNA.

References