Difference between revisions of "Part:BBa K1456001:Design"
(→‎Cloning)
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<center>[[File:ATOMS-tpa_results1.png|400px|]]</center><br/>
 
<center>[[File:ATOMS-tpa_results1.png|400px|]]</center><br/>
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== '''Cloning''' ==
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===Cloning===
  
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[[File:https://static.igem.org/mediawiki/2014/f/f4/ATOMS-tpa_results3.png]]
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<center>[[File:ATOMS-tpa_results3.png|400px|]]<center/>
  
 
*Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.
 
*Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.

Revision as of 14:14, 21 October 2014

Human Tissue Plasminogen Activator (htPA)

ATOMS-tpa results1.png

Cloning

ATOMS-tpa results3.png<center/>
  • Through the primers that we ordered and the cDNA, we acquired tPA inserts. The insert was, as expected, portraying that the base length was around 1700 bp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 958


Design Notes

None

Source

We clonned this part from human hepatocellular carcinoma cell genome cDNA.

===References===