Difference between revisions of "Part:BBa K1321326"
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<partinfo>BBa_K1321326 short</partinfo>
 
<partinfo>BBa_K1321326 short</partinfo>
  
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This is a blue chromoprotein aeBlue (BBa_K1033280) expressed in pSEVA331-Bb plasmid backbone (part BBa_K1321300). This construct is a member of the ''G.xylinus'' genetic engineering toolbox.  
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This is a blue chromoprotein aeBlue (BBa_K1033280) expressed in pSEVA331-Bb plasmid backbone (part BBa_K1321300). This construct is a member of the ''G.xylinus'' genetic engineering toolkit (parts BBa_K1321295 - BBa_K1321332).
  
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''G.xylinus'' toolbox was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium ''Gluconacetobacter xylinus'' (parts Bba_K1321305 and BBa_K1321306) by characterizing and providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in ''G.xylinus'' and ''E.coli'' (a shuttle vector) and was selected as the registry's standard plasmid backbone pSB1C3 can not be used for ''G.xylinus'' engineering.
 
  
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NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in ''Gluconacetobacter'' species, the ''G.xylinus'' genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the registry. However, in order to make the ''G.xylinus'' toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Imperial iGEM 2014 team.
 
  
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''G.xylinus'' toolkit was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium ''Gluconacetobacter xylinus'' (parts Bba_K1321305 and BBa_K1321306). As no registry parts had been tested in ''G.xylinus'', the aim of this toolkit was to determine the parts usable in ''G.xylinus'' and to characterize them in this host. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in ''G.xylinus'' and ''E.coli'' (a shuttle vector) and was selected because the registry's standard plasmid backbone pSB1C3 can not be used for ''G.xylinus'' engineering.
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NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in ''Gluconacetobacter'' species, the ''G.xylinus'' genetic engineering toolkit is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the Registry. However, in order to make the ''G.xylinus'' toolkit available for the synthetic biology community, Imperial iGEM 2014 team has made it freely available upon request, with quality control provided (see Experience). To request, please contact Imperial iGEM 2014 team.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 23:45, 20 October 2014

pTet01

This is a blue chromoprotein aeBlue (BBa_K1033280) expressed in pSEVA331-Bb plasmid backbone (part BBa_K1321300). This construct is a member of the G.xylinus genetic engineering toolkit (parts BBa_K1321295 - BBa_K1321332).



G.xylinus toolkit was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306). As no registry parts had been tested in G.xylinus, the aim of this toolkit was to determine the parts usable in G.xylinus and to characterize them in this host. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected because the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.

NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolkit is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the Registry. However, in order to make the G.xylinus toolkit available for the synthetic biology community, Imperial iGEM 2014 team has made it freely available upon request, with quality control provided (see Experience). To request, please contact Imperial iGEM 2014 team. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4685
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4685
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
    Illegal NotI site found at 1822
    Illegal NotI site found at 4691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4685
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4685
    Illegal XbaI site found at 4700
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
    Illegal NgoMIV site found at 3004
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    COMPATIBLE WITH RFC[1000]