Difference between revisions of "Part:BBa K1321326:Design"
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<partinfo>BBa_K1321326 short</partinfo> | <partinfo>BBa_K1321326 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | BBa_K1321302 was created by restricting BBa_K1033280 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Revision as of 21:47, 20 October 2014
pTet01
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4685
Illegal SpeI site found at 946
Illegal PstI site found at 1829 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4685
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 946
Illegal PstI site found at 1829
Illegal NotI site found at 1822
Illegal NotI site found at 4691 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4685
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4685
Illegal SpeI site found at 946
Illegal PstI site found at 1829 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4685
Illegal XbaI site found at 4700
Illegal SpeI site found at 946
Illegal PstI site found at 1829
Illegal NgoMIV site found at 3004
Illegal AgeI site found at 1525
Illegal AgeI site found at 1637 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa_K1321302 was created by restricting BBa_K1033280 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.