Difference between revisions of "Part:BBa K1321325:Design"
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<partinfo>BBa_K1321325 short</partinfo> | <partinfo>BBa_K1321325 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | BBa_K1321302 was created by restricting BBa_K1073022 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing. | |
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===References=== | ===References=== | ||
Revision as of 21:46, 20 October 2014
pLux02
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4802
Illegal SpeI site found at 1063
Illegal PstI site found at 1946 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4802
Illegal SpeI site found at 1063
Illegal PstI site found at 1946
Illegal NotI site found at 1939
Illegal NotI site found at 4808 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4802
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4802
Illegal SpeI site found at 1063
Illegal PstI site found at 1946 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4802
Illegal XbaI site found at 4817
Illegal SpeI site found at 1063
Illegal PstI site found at 1946
Illegal NgoMIV site found at 3121
Illegal AgeI site found at 1642
Illegal AgeI site found at 1754 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Design Notes
BBa_K1321302 was created by restricting BBa_K1073022 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.