Difference between revisions of "Part:BBa K1321324:Design"
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<partinfo>BBa_K1321324 short</partinfo> | <partinfo>BBa_K1321324 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | BBa_K1321302 was created by restricting BBa_K1033903 (Anderson promoter+RFP insert) and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Revision as of 21:45, 20 October 2014
pLux01
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4785
Illegal SpeI site found at 1046
Illegal PstI site found at 1929 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4785
Illegal SpeI site found at 1046
Illegal PstI site found at 1929
Illegal NotI site found at 1922
Illegal NotI site found at 4791 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4785
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4785
Illegal SpeI site found at 1046
Illegal PstI site found at 1929 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4785
Illegal XbaI site found at 4800
Illegal SpeI site found at 1046
Illegal PstI site found at 1929
Illegal NgoMIV site found at 3104
Illegal AgeI site found at 1625
Illegal AgeI site found at 1737 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987
Design Notes
BBa_K1321302 was created by restricting BBa_K1033903 (Anderson promoter+RFP insert) and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.