Difference between revisions of "Part:BBa K1456002:Experience"
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The amounts of protein acquired were as the charts above and the these data were taken into consideration in the preparation of the assays. | The amounts of protein acquired were as the charts above and the these data were taken into consideration in the preparation of the assays. | ||
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| + | https://static.igem.org/mediawiki/2014/d/df/ATOMS_gpx_results_17.jpg | ||
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| + | A '''functional assay''' of GPx was done '''through NADPH to measure GPx activity'''. The same amount of genes were transferred to all samples but varying amount of Na2SeO3 was prepared in the samples, with 2 samples containing different Na2SeO3 concentrations and one control group. The GPx gene was not transfected to the control group. From these 3 groups, the assay of the control group gave the lowest yield as expected; the sample of 500 nM Na2SeO3 produced the mid-result. The sample of 1000 nM Na2SeO3 yielded the highest activity results in the assay. These results prove that the GPx production is directly proportional to the amount of added Na2Se2O3 and that the GPx enzyme was produced functionally. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:34, 20 October 2014
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Applications of BBa_K1456002
ATOMS TURKİYE 2014
The amounts of protein acquired were as the charts above and the these data were taken into consideration in the preparation of the assays.
A functional assay of GPx was done through NADPH to measure GPx activity. The same amount of genes were transferred to all samples but varying amount of Na2SeO3 was prepared in the samples, with 2 samples containing different Na2SeO3 concentrations and one control group. The GPx gene was not transfected to the control group. From these 3 groups, the assay of the control group gave the lowest yield as expected; the sample of 500 nM Na2SeO3 produced the mid-result. The sample of 1000 nM Na2SeO3 yielded the highest activity results in the assay. These results prove that the GPx production is directly proportional to the amount of added Na2Se2O3 and that the GPx enzyme was produced functionally.
User Reviews
UNIQ9967273432d6ea6f-partinfo-00000000-QINU UNIQ9967273432d6ea6f-partinfo-00000001-QINU