Difference between revisions of "Part:BBa K1321318"

Revision as of 15:47, 20 October 2014

Anderson J23114-RFP in pSEVA331-Bb

This is Anderson promoter J23114 driving the expression of Elowitz RBS-mFRP in pSEVA331-Bb plasmid backbone (part BBa_K1321300 and is a member of the G.xylinus genetic engineering toolbox.

G.xylinus toolbox was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306) by providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected as the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.

NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which can't be quality controlled and thus maintained by the registry. However, in order to make the G.xylinus toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Michael Florea at mf1512@imperial.ac.uk.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 3776
Illegal SpeI site found at 37
Illegal PstI site found at 920
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3776
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NotI site found at 913
Illegal NotI site found at 3782
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3776
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 3776
Illegal SpeI site found at 37
Illegal PstI site found at 920
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 3776
Illegal XbaI site found at 3791
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NgoMIV site found at 2095
Illegal AgeI site found at 616
Illegal AgeI site found at 728
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321318 short</partinfo>
-x
+This is Anderson promoter '''J23114''' driving the expression of Elowitz RBS-mFRP in pSEVA331-Bb plasmid backbone (part BBa_K1321300 and is a member of the ''G.xylinus'' genetic engineering toolbox.
+''G.xylinus'' toolbox was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium'' Gluconacetobacter xylinus'' (parts Bba_K1321305 and BBa_K1321306) by providing a collection of widely used parts in pSEVA331-Bb backbone. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in ''G.xylinus'' and ''E.coli'' (a shuttle vector) and was selected as the registry's standard plasmid backbone pSB1C3 can not be used for ''G.xylinus'' engineering.
+NOTE:
+Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in ''Gluconacetobacter'' species, the ''G.xylinus'' genetic engineering toolbox is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which can't be quality controlled and thus maintained by the registry. However, in order to make the ''G.xylinus'' toolbox available for the synthetic biology community, Imperial iGEM 2014 team has made the toolbox freely available upon request, with quality control provided (see Experience). For requests, please contact Michael Florea at mf1512@imperial.ac.uk.
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