Difference between revisions of "Part:BBa K1415207"
Alex19950425 (Talk | contribs) |
Alex19950425 (Talk | contribs) |
||
(8 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
<partinfo>BBa_K1415207 short</partinfo> | <partinfo>BBa_K1415207 short</partinfo> | ||
− | We | + | To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli. |
+ | |||
+ | |||
+ | |||
+ | [[File:ALLAS.png|thumb|center|900px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(AS) + BFP + Term.]] | ||
+ | [[File:BLAS.png|thumb|center|1200px|'''Fig.1-2''' The bluelight test of our E.coli]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | [[File:PBAN Fluorescence Value (1).jpg|thumb|center|650px|'''Fig.1-3''' The blue light fluorescence expression curve of E.coli containing Pcons + RBS + 9 different kinds of PBAN + RBS + BFP + Ter plasmid (control: competent cells that cannot emit blue light). Below are biobrick serial numbers of the PBAN abbreviations: | ||
+ | SL: BBa_K1415205 BM: BBa_K1415201 MB: BBa_K1415202 | ||
+ | AI: BBa_K1415203 LD: BBa_K1415204 HAH:BBa_K1415206 | ||
+ | AS: BBa_K1415207 SI: BBa_K1415208 AA: BBa_K1415209]] | ||
+ | |||
+ | |||
+ | |||
+ | <p style="font-size:120%">'''Modeling'''</p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | [[File:MDAS.png|thumb|center|1200px|'''Fig.1-4''' Modeling result of Pcons + RBS + PBAN(AS) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(AS) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.]] | ||
+ | |||
+ | |||
+ | |||
Line 9: | Line 39: | ||
<!-- --> | <!-- --> | ||
− | + | ||
<partinfo>BBa_K1415207 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1415207 SequenceAndFeatures</partinfo> | ||
Line 17: | Line 47: | ||
<partinfo>BBa_K1415207 parameters</partinfo> | <partinfo>BBa_K1415207 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
− | |||
− |
Latest revision as of 14:44, 20 October 2014
Pcons+B0034+PBAN(Adoxophyes sp.)+B0034+BFP+J61048
To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.
Modeling
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 903
- 1000COMPATIBLE WITH RFC[1000]