Difference between revisions of "Part:BBa K1493702"
| Line 5: | Line 5: | ||
This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493703 in a pSB3K3 backbone. | This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493703 in a pSB3K3 backbone. | ||
| − | + | https://static.igem.org/mediawiki/2014/5/5a/Characterization_Toggle_Switch.png | |
‘’Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).’’ | ‘’Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).’’ | ||
Revision as of 10:28, 20 October 2014
Toggle switch part with reporter gene gfp
Part of the BananaGuard toggle switch containing a gfp reporter gene. Combined with the BioBrick BBa_K1493703 this part forms a toggle switch. This BioBrick is tested as the whole toggle switch. The part is assembled with BBa_K1493703 in a pSB3K3 backbone.
‘’Figure 1. The relative fluorescence unit of each toggle switch state. Fluorescence is measured in duplo of cell cultures carrying the pSB3K3 plasmid with the toggle switch construct (BBa_K1493702, BBa_K1493703) grown in M9 medium containing 500 ng/ml aTc (green), 2 mM IPTG (red) and with no inducer added to the medium (blue).’’
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1227
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1903