Difference between revisions of "Part:BBa K1321334"
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<partinfo>BBa_K1321334 short</partinfo> | <partinfo>BBa_K1321334 short</partinfo> | ||
| − | This part is made up of the | + | This part is made up of the coding sequence of the Cellulose Synthase enzyme, which has been codon-optimised for expression in <em>Escherichia coli</em>. In <em>Gluconacetobacter xylinus</em>, this is contained within the Acs cellulose synthesis operon, a functional unit containing the essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such fundamental enzymes in Cellulose Production. It is made up of two main domains, a regulatory domain and a catalytic domain. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:18, 20 October 2014
pSB-AcsAB
This part is made up of the coding sequence of the Cellulose Synthase enzyme, which has been codon-optimised for expression in Escherichia coli. In Gluconacetobacter xylinus, this is contained within the Acs cellulose synthesis operon, a functional unit containing the essential elements for cellulose assembly and secretion to the extracellular space. Cellulose synthase is one of such fundamental enzymes in Cellulose Production. It is made up of two main domains, a regulatory domain and a catalytic domain. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1831
Illegal BglII site found at 2545
Illegal BglII site found at 4468
Illegal BamHI site found at 841
Illegal BamHI site found at 3835 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1817
Illegal AgeI site found at 1894
Illegal AgeI site found at 2450
Illegal AgeI site found at 3251
Illegal AgeI site found at 3343
Illegal AgeI site found at 3449 - 1000COMPATIBLE WITH RFC[1000]