Difference between revisions of "Part:BBa K1413043:Design"

 
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<partinfo>BBa_K1413043 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
 
This part follows rules of RFC92, compatible with RFC10.
 
This part follows rules of RFC92, compatible with RFC10.
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The complex Tn10/IS10 is involved in the non-replicative cut-and-paste mechanism. The transposable segment is excised at its ends and is then re-inserted randomly in a DNA site.
  
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The Tn10 transposase protein is made of 402 amino-acids, which recognises inverted repeats insertion sequence; Is10-right and Is10-left. The Tn10 protein expression is strongly regulated by various positive and negative regulation mechanisms.
  
  

Revision as of 19:03, 19 October 2014

Transposase Tn10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 244
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part follows rules of RFC92, compatible with RFC10. The complex Tn10/IS10 is involved in the non-replicative cut-and-paste mechanism. The transposable segment is excised at its ends and is then re-inserted randomly in a DNA site.

The Tn10 transposase protein is made of 402 amino-acids, which recognises inverted repeats insertion sequence; Is10-right and Is10-left. The Tn10 protein expression is strongly regulated by various positive and negative regulation mechanisms.


Source

This part was given by a member of the institute of systems and synthetic biology (Evry, France)

References