Difference between revisions of "Part:BBa K1462502"
 
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<partinfo>BBa_K1462502 short</partinfo>
 
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The PDZ domain + linker is the fusion unit of the scaffold protein and help the targeted emzyme to bind on. So we added the linker behind it and help do the next job.
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The PDZ domain, one of our scaffold subunits, is a part of the α-syntrophin protein in Mus musculus. Now we do the codon opmitization according to our host cell S.cerevisae. The PDZ domain allows the assembly of our targeted protein CA. And the domain is the third subunit of our scaffold protein.
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[[File:Gbd.png|200px|left|frame|Simulative structure of the PDZ domain which we add the linker behind it.]]
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'></span>
 
<partinfo>BBa_K1462502 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1462502 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:19, 19 October 2014

PDZ domain + Linker

The PDZ domain, one of our scaffold subunits, is a part of the α-syntrophin protein in Mus musculus. Now we do the codon opmitization according to our host cell S.cerevisae. The PDZ domain allows the assembly of our targeted protein CA. And the domain is the third subunit of our scaffold protein.

Simulative structure of the PDZ domain which we add the linker behind it.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 310
    Illegal XhoI site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]