Difference between revisions of "Part:BBa K1462501"
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<partinfo>BBa_K1462501 short</partinfo>
 
<partinfo>BBa_K1462501 short</partinfo>
  
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The GBD domain + linker is the fusion unit of the scaffold protein and help the targeted emzyme to bind on. So we added the linker behind it and help do the next job.
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The GBD domain, one of our scaffold subunits, is a part of GTPase binding domain in Rattus rattus. Now we do the codon opmitization according to our host cell S.cerevisae. The GBD domain allows the assembly of our targeted protein PRK. And the domain is the first subunit of our scaffold protein.
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[[File:Gbd.png|200px|left|frame|Figure 1. The GBD domain added linker ]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:32, 19 October 2014

GBD domian + Linker

The GBD domain, one of our scaffold subunits, is a part of GTPase binding domain in Rattus rattus. Now we do the codon opmitization according to our host cell S.cerevisae. The GBD domain allows the assembly of our targeted protein PRK. And the domain is the first subunit of our scaffold protein.

Figure 1. The GBD domain added linker

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 60
    Illegal BamHI site found at 93
    Illegal BamHI site found at 265
    Illegal XhoI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]