Difference between revisions of "Part:BBa K1529320"

 
 
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<partinfo>BBa_K1529320 short</partinfo>
 
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We newly developed the Prhl(RR) promoter (<partinfo>BBa_K1529320</partinfo>) by improving Prhl promoter (<partinfo>BBa_R0071</partinfo>) which is activated by C4HSL-RhlR complex (Fig. 1). <br>
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To characterize the function of this Prhl(RR) promoter, we constructed a part, Prhl(RR)-GFP (<partinfo>BBa_K1529301</partinfo>), by inserting the Prhl(RR) promoter, upstream of a GFP coding sequence.
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[[Image:titech2014_parts_rhl_promoter_main_Fig1.png|thumb|center|550px|<b>Fig. 1.</b> Our newly designed  promoter]]
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[[Image:titech2014_parts_luxR_and_rhlR_difference_main_Fig2.png|thumb|center|550px|<b>Fig. 2.</b> Difference between LuxR and RhlR]]
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By using the reporter cells that contain Prhl(RR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.) <br>
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We saw that our new Prhl(RR) promoter was actually activated by C4HSL through an induction assay
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[[Image:Improved_Prhl(RR)_Promoter_Assay_Result.png|thumb|center|600px|<b>Fig. 3.</b> Fluorescence intensity detected by flow cytometer]]
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For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].
  
 
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Latest revision as of 11:52, 19 October 2014

Prhl(RR)

We newly developed the Prhl(RR) promoter (BBa_K1529320) by improving Prhl promoter (BBa_R0071) which is activated by C4HSL-RhlR complex (Fig. 1).
To characterize the function of this Prhl(RR) promoter, we constructed a part, Prhl(RR)-GFP (BBa_K1529301), by inserting the Prhl(RR) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter
Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(RR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.)
We saw that our new Prhl(RR) promoter was actually activated by C4HSL through an induction assay

Fig. 3. Fluorescence intensity detected by flow cytometer


For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]