Difference between revisions of "Part:BBa K1529310"

Revision as of 11:47, 19 October 2014

Prhl(LR)

We newly developed the Prhl(LR) promoter (BBa_K1529310) by improving Prhl promoter (BBa_R0071) which is activated by C4HSL-RhlR complex (Fig. 1).
To characterize the function of this Prhl(LR) promoter, we constructed a part, Prhl(LR)-GFP (BBa_K1529311), by inserting the Prhl(LR) promoter, upstream of a GFP coding sequence.

Fig. 1. Our newly designed promoter

Fig. 2. Difference between LuxR and RhlR

By using the reporter cells that contain Prhl(LR)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. (Fig. 3.)
We saw that our new Prhl(LR) promoter was actually activated by C4HSL through an induction assay

Fig. 3. Fluorescence intensity detected by flow cytometer

For more information, see [http://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay our work in Tokyo_Tech 2014 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1529310 short</partinfo>
-We newly developed the Prhl(LR) promoter (<partinfo>BBa_K1529310</partinfo>) by improving Prhl promoter (<partinfo>BBa_R0071</partinfo>) which is activated by C4HSL-RHLR complex  (Fig. 1). <br>
+We newly developed the Prhl(LR) promoter (<partinfo>BBa_K1529310</partinfo>) by improving Prhl promoter (<partinfo>BBa_R0071</partinfo>) which is activated by C4HSL-RhlR complex  (Fig. 1). <br>
 To characterize the function of this Prhl(LR) promoter, we constructed a part, Prhl(LR)-GFP (<partinfo>BBa_K1529311</partinfo>), by inserting the Prhl(LR) promoter, upstream of a GFP coding sequence.