Difference between revisions of "Part:BBa K1362401:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part represents only the intein sequence without | + | This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs. |
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| − | + | ||
===Source=== | ===Source=== | ||
| − | Obtained from pVS41 by Prof. Henning D. Mootz, University of Muenster. | + | Obtained by PCR amplification from pVS41 by Prof. Henning D. Mootz, University of Muenster.[[#References|[1]]] |
===References=== | ===References=== | ||
| + | |||
| + | [1] Joachim Zettler, Vivien Schütz, Henning D. Mootz, The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction, FEBS Letters, Volume 583, Issue 5, 4 March 2009, Pages 909-914, ISSN 0014-5793, http://dx.doi.org/10.1016/j.febslet.2009.02.003. | ||
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| + | [2] Cheriyan, M., Pedamallu, C. S., Tori, K. & Perler, F. Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues. J. Biol. Chem. 288, 6202–11 (2013). | ||
Latest revision as of 10:38, 19 October 2014
NpuDnaE C-Intein cloning piece
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Obtained by PCR amplification from pVS41 by Prof. Henning D. Mootz, University of Muenster.[1]
References
[1] Joachim Zettler, Vivien Schütz, Henning D. Mootz, The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction, FEBS Letters, Volume 583, Issue 5, 4 March 2009, Pages 909-914, ISSN 0014-5793, http://dx.doi.org/10.1016/j.febslet.2009.02.003.
[2] Cheriyan, M., Pedamallu, C. S., Tori, K. & Perler, F. Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues. J. Biol. Chem. 288, 6202–11 (2013).