Difference between revisions of "Part:BBa K1331004"

Latest revision as of 08:53, 19 October 2014

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

This is an improvement of BBa_K317999 designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.

RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. This part encodes rhamnosyltransferase I subunit B, and our part BBa_K1331001 encodes subunit A.

The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB (BBa_K1331006) and rhlABRI (BBa_K1331005).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 871
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 387

@@ Line 8: / Line 8: @@
 The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.
-RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part encodes rhamnosyltransferase I subunit B, and the other part [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] encodes subunit A.
+RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part encodes rhamnosyltransferase I subunit B, and our part [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] encodes subunit A.
-The noncoding sequence included in this part is for composition with rhlA and rhlR. See our composite part rhlABRI ([https://parts.igem.org/Part:BBa_K1331005 BBa_K1331005]).
+The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB ([https://parts.igem.org/Part:BBa_K1331006 BBa_K1331006]) and rhlABRI ([https://parts.igem.org/Part:BBa_K1331005 BBa_K1331005]).