Difference between revisions of "Part:BBa K1331004"
 
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<partinfo>BBa_K1331004 short</partinfo>
 
<partinfo>BBa_K1331004 short</partinfo>
  
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RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part includes a part of upstream noncoding gene and a part of downstreanm noncoding gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
 
  
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This is an improvement of [https://parts.igem.org/Part:BBa_K317999 BBa_K317999] designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
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===Usage and Biology===
 
===Usage and Biology===
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The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.
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RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part encodes rhamnosyltransferase I subunit B, and our part [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] encodes subunit A.
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The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB ([https://parts.igem.org/Part:BBa_K1331006 BBa_K1331006]) and rhlABRI ([https://parts.igem.org/Part:BBa_K1331005 BBa_K1331005]).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1331004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1331004 SequenceAndFeatures</partinfo>

Latest revision as of 08:53, 19 October 2014

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6


This is an improvement of BBa_K317999 designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.

RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. This part encodes rhamnosyltransferase I subunit B, and our part BBa_K1331001 encodes subunit A.

The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB (BBa_K1331006) and rhlABRI (BBa_K1331005).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387