Difference between revisions of "Part:BBa K1331004"

 
 
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<partinfo>BBa_K1331004 short</partinfo>
 
<partinfo>BBa_K1331004 short</partinfo>
  
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RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
 
  
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This is an improvement of [https://parts.igem.org/Part:BBa_K317999 BBa_K317999] designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
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===Usage and Biology===
 
===Usage and Biology===
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The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.
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RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''. This part encodes rhamnosyltransferase I subunit B, and our part [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] encodes subunit A.
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The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB ([https://parts.igem.org/Part:BBa_K1331006 BBa_K1331006]) and rhlABRI ([https://parts.igem.org/Part:BBa_K1331005 BBa_K1331005]).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1331004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1331004 SequenceAndFeatures</partinfo>

Latest revision as of 08:53, 19 October 2014

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6


This is an improvement of BBa_K317999 designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.

RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. This part encodes rhamnosyltransferase I subunit B, and our part BBa_K1331001 encodes subunit A.

The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB (BBa_K1331006) and rhlABRI (BBa_K1331005).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387