Difference between revisions of "Part:BBa K1331007"
 
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__NOTOC__
 
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<partinfo>BBa_S05247 short</partinfo>
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<partinfo>BBa_K1331007 short</partinfo>
  
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This part is combined with [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002] and [https://parts.igem.org/Part:BBa_K1331003 BBa_K1331003].
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This part encodes both rhlR and rhlI. It is composited with [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002] and [https://parts.igem.org/Part:BBa_K1331003 BBa_K1331003].
  
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It includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.
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===Usage and Biology===
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The sequence we provide here includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.
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The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.
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The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system.
 
 
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[[Image:NKrhlR_PCR.jpg‎]]
 
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Image:E0040_invitro_calibration.png<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_S05247 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1331007 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_S05247 parameters</partinfo>
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<partinfo>BBa_K1331007 parameters</partinfo>
 
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Latest revision as of 04:53, 19 October 2014

Modified rhlRI coding sequence from Pseudomonas aeruginosa SQ6

This part encodes both rhlR and rhlI. It is composited with BBa_K1331002 and BBa_K1331003.

Usage and Biology

The sequence we provide here includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.

The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
    Illegal BamHI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1126