Difference between revisions of "Part:BBa K1331003"
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The noncoding sequence included in this part is for composition with our part [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002] | The noncoding sequence included in this part is for composition with our part [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002] | ||
| − | . See our composite part rhlRI ([ | + | . See our composite part rhlRI ([https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]). |
Latest revision as of 04:44, 19 October 2014
RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6
This part encodes rhlI, an acyl-homoserine-lactone synthase in Pseudomonas aeruginosa.
The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.
Usage and Biology
The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.
RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.
The noncoding sequence included in this part is for composition with our part BBa_K1331002 . See our composite part rhlRI (BBa_K1331007).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 89
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 221