Difference between revisions of "Part:BBa K1331003"

Latest revision as of 04:44, 19 October 2014

RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6

This part encodes rhlI, an acyl-homoserine-lactone synthase in Pseudomonas aeruginosa.

The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.

RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.

The noncoding sequence included in this part is for composition with our part BBa_K1331002 . See our composite part rhlRI (BBa_K1331007).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 89
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 221

@@ Line 3: / Line 3: @@
 This part encodes rhlI, an acyl-homoserine-lactone synthase in ''Pseudomonas aeruginosa''.
 The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.
@@ Line 12: / Line 13: @@
 The noncoding sequence included in this part is for composition with our part [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002]
-. See our composite part rhlRI ([http://https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).
+. See our composite part rhlRI ([https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).