Difference between revisions of "Part:BBa K1331003"

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This part encodes rhlI, an acyl-homoserine-lactone synthase in ''Pseudomonas aeruginosa''.  
 
This part encodes rhlI, an acyl-homoserine-lactone synthase in ''Pseudomonas aeruginosa''.  
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The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.
 
The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.
  
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The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.
 
The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.
  
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RhlI is an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR. RhlI is required for the synthesis of autoinducer molecules C4-HSL.Together with rhlR positively regulates the promoter of rhlAB.
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RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.
  
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The noncoding sequence included in this part is for composition with rhlR. See our composite part rhlRI ([http://https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).
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The noncoding sequence included in this part is for composition with our part [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002]
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. See our composite part rhlRI ([https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).
  
  

Latest revision as of 04:44, 19 October 2014

RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6

This part encodes rhlI, an acyl-homoserine-lactone synthase in Pseudomonas aeruginosa.

The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.

RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.

The noncoding sequence included in this part is for composition with our part BBa_K1331002 . See our composite part rhlRI (BBa_K1331007).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221