Difference between revisions of "Part:BBa K1331003"

 
(→‎Usage and Biology)
 
(9 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1331003 short</partinfo>
 
<partinfo>BBa_K1331003 short</partinfo>
  
−
This sequece encodes RhlI, an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR.
+
This part encodes rhlI, an acyl-homoserine-lactone synthase in ''Pseudomonas aeruginosa''.
 +
 
 +
The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.
 +
 +
RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.
 +
 +
The noncoding sequence included in this part is for composition with our part [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002]
 +
. See our composite part rhlRI ([https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 04:44, 19 October 2014

RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6

This part encodes rhlI, an acyl-homoserine-lactone synthase in Pseudomonas aeruginosa.

The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.

RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.

The noncoding sequence included in this part is for composition with our part BBa_K1331002 . See our composite part rhlRI (BBa_K1331007).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221