Difference between revisions of "Part:BBa K1403003:Design"

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===References===
 
===References===
  
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iGEM07_Edinburgh: https://parts.igem.org/Part:BBa_I742110
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[https://parts.igem.org/Part:BBa_I742110 iGEM 2007 Edinburgh]
  
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Salis Lab. RBS calculator: https://salis.psu.edu/software/forward
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[https://salis.psu.edu/software/forward Salis Lab RBS Calculator]
 
H.M Salis, Methods in Enzymology 2011
 
H.M Salis, Methods in Enzymology 2011
 
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009
 
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009

Revision as of 04:19, 19 October 2014

Limonene synthase (LIMS1) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

The purpose of this part is to produce limonene in E. coli.

We used LIMS1 CDS from part BBa_I742110 and inserted constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.

Source

BBa_I742110

References

iGEM 2007 Edinburgh

Salis Lab RBS Calculator H.M Salis, Methods in Enzymology 2011 H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009