Difference between revisions of "Part:BBa B0011"

Line 1: Line 1:
<SPAN style='position:absolute;font-size:90%;position:relative;top:-15px'>Designed by <partinfo>B0011 designer</partinfo></SPAN>
 
<SPAN style='position:absolute;float:right;font-size:90%;position:relative;top:-15px'>Entered: <partinfo>B0011 entrydate</partinfo></SPAN>
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>B0011 short</partinfo>
 
<partinfo>B0011 short</partinfo>

Revision as of 20:51, 29 April 2006

LuxICDABEG (+/-)

Bidirectional transcriptional terminator consisting of a 22bp stem-loop.

Usage and Biology

Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the stem loop. Also, it is found between two coding regions that point towards each other.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

biologyLuxIA
directionBidirectional
forward_efficiency0.419[CC]/0.95[JK]
n/aForward
reversed_version585
reverse_efficiency0.636[CC]/0.86[JK]