Difference between revisions of "Part:BBa K1331004"
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<partinfo>BBa_K1331004 short</partinfo> | <partinfo>BBa_K1331004 short</partinfo> | ||
| − | This is an improvement of [https://parts.igem.org/Part:BBa_K317999 | + | |
| + | This is an improvement of [https://parts.igem.org/Part:BBa_K317999 BBa_K317999] designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 04:10, 19 October 2014
Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
This is an improvement of BBa_K317999 designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
Usage and Biology
The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.
RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. This part encodes rhamnosyltransferase I subunit B, and the other part BBa_K1331001 encodes subunit A.
The noncoding sequence included in this part is for composition with rhlA (BBa_K1331001) and rhlR (BBa_K1331002). See our composite part rhlABRI (BBa_K1331005).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 871 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 387