Difference between revisions of "Part:BBa K1331001"

Revision as of 03:40, 19 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This is an improvement of BBa_K317998 designed by iGEM10_Tokyo-NoKoGen. A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.

RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.This part encodes rhamnosyltransferase I subunit A, and the other part BBa_K1331004 encodes subunit B.

The noncoding sequence included in this part is for composition with rhlB. The downstream noncoding sequence of rhlA and The upstream noncoding sequence of rhlB (BBa_K1331004) make the whole noncoding sequence between rhlA and rhlB in Pseudomonas aeruginosa, preserving the overall function of the compositive part. We also built this compositive part rhlAB, see BBa_K1331006.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA. RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004] encodes subunit B.
+The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.
+RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004] encodes subunit B.
 The noncoding sequence included in this part is for composition with rhlB. The downstream noncoding sequence of rhlA and The upstream noncoding sequence of rhlB ([https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004]) make the whole noncoding sequence between rhlA and rhlB in ''Pseudomonas aeruginosa'', preserving the overall function of the compositive part. We also built this compositive part rhlAB, see [https://parts.igem.org/Part:BBa_K1331006 BBa_K1331006].