Difference between revisions of "Part:BBa K1403017:Design"

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===Source===
 
===Source===
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Non-redundant <i>Lactococcus lactis</i> AldB from [http://www.ncbi.nlm.nih.gov/protein/500160484?report=genbank&log$=protalign&blast_rank=1&RID=46AXW03K01R GenBank:U00096.3].
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Non-redundant <i>Lactococcus lactis</i> AldB from [http://www.ncbi.nlm.nih.gov/protein/500160484?report=genbank&log$=protalign&blast_rank=1&RID=46AXW03K01R NCBI: WP_011835154.1].
  
 
===References===
 
===References===

Revision as of 03:36, 19 October 2014

Acetaldehyde dehydrogenase (aldB) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purpose of this part is to biosynthesize acetoin using E. coli. The sequence was synthesized as a gBlock by IDT with the codon optimized aldB downstream of constitutive promoter BBa_J23108 and a sRBS. Alpha-acetolactate decarboxylase transforms acetolactate to acetoin, which has butter scent.

Source

Non-redundant Lactococcus lactis AldB from [http://www.ncbi.nlm.nih.gov/protein/500160484?report=genbank&log$=protalign&blast_rank=1&RID=46AXW03K01R NCBI: WP_011835154.1].

References

Anderson Promoter Collection

David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.

Salis lab RBS calculator
H.M Salis, Methods in Enzymology 2011
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009