Difference between revisions of "Part:BBa K1403003:Design"

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===Design Notes===
 
===Design Notes===
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The purpose of this plasmid is to produce limonene in <i>E.coli</i>.   
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The purpose of this part is to produce limonene in <i>E.coli</i>.   
  
 
We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS.  Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
 
We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS.  Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

Revision as of 01:36, 19 October 2014

Limonene synthase (LIMS1) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

The purpose of this part is to produce limonene in E.coli.

We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.

Source

Registry. BBa_I742110

References

iGEM07_Edinburgh: https://parts.igem.org/Part:BBa_I742110

Salis Lab. RBS calculator: https://salis.psu.edu/software/forward H.M Salis, Methods in Enzymology 2011 H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009