Difference between revisions of "Part:BBa K1403017"
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Transform <i>E. coli</i> to produce acetoin. | Transform <i>E. coli</i> to produce acetoin. | ||
The enzyme requires alpha acetolactate. | The enzyme requires alpha acetolactate. | ||
| − | A co-transformation with part BBa_K1403019 increases the amount of substrate for | + | A co-transformation with part BBa_K1403019 increases the amount of substrate for AldB. |
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Revision as of 01:32, 19 October 2014
Acetaldehyde dehydrogenase (aldB) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: AldB from Lactococcus lactis
- Terminators BBa_B0010 & BBa_B0012
Transform E. coli to produce acetoin. The enzyme requires alpha acetolactate. A co-transformation with part BBa_K1403019 increases the amount of substrate for AldB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Verification
Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter