Difference between revisions of "Part:BBa K1403003:Design"

Revision as of 23:49, 18 October 2014

Limonene synthase (LIMS1) expression cassette

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 82
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 187

Design Notes

The purpose of this plasmid is to produce limonene in E.coli.

We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.

Source

Registry. BBa_I742110

References

iGEM07_Edinburgh: https://parts.igem.org/Part:BBa_I742110

Salis Lab. RBS calculator: https://salis.psu.edu/software/forward H.M Salis, Methods in Enzymology 2011 H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009

@@ Line 8: / Line 8: @@
 The purpose of this plasmid is to produce limonene in <i>E.coli</i>.
-We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS.  Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
+We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS.  Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
 The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.