Difference between revisions of "Part:BBa K1502001:Design"
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===Source=== | ===Source=== | ||
| − | This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene. | + | This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene (#49809). |
===References=== | ===References=== | ||
(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598. | (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598. | ||
Latest revision as of 23:47, 18 October 2014
accA portion of Acyl-CoA Ligase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 579
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 517
Illegal SapI.rc site found at 714
Design Notes
The PCR process included X and P overhangs in order to include into the pSB1C3 plasmid.
Source
This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene (#49809).
References
(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.