Difference between revisions of "Part:BBa K1403003:Design"

Revision as of 23:34, 18 October 2014

Limonene synthase (LIMS1) expression cassette

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 82
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 187

Design Notes

The purpose of this plasmid is to produce limonene in E.coli.

We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.

Source

Registry. BBa_I742110

References

Edinburg iGEM Project 2007

@@ Line 6: / Line 6: @@
 ===Design Notes===
-The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.
+The purpose of this plasmid is to produce limonene in <i>E.coli</i>.
-We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
+We used LIMS1 CDS from part BBa_I742110 and inserted a constitutive promoter BBa_J23108 and a sRBS upstream of the CDS.  Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
-The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format.
+The promoter and RBS were designed as oligos, aligned, digested and ligated. The finished plasmid is in standard biobricks format.
 ===Source===