Difference between revisions of "Part:BBa K1526005"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1526005 short</partinfo>
 
<partinfo>BBa_K1526005 short</partinfo>
  
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LacI + Gsh1
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Lac Repressor System + Gsh1
  
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<!-- Add more about the biology of this part here
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<b>Original Gene:</b> gsh1/gshA<br>
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===Usage and Biology===
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<b>Organism:</b> <i>Saccharomyces cerevisiae</i><br>
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<b>Protein:</b> Glutamate-Cysteine Ligase (previously known as gamma-glutamylcysteine synthetase)<br>
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<b>Function:</b> Gamma glutamylcysteine synthetase catalyzes the first step in glutathione (GSH) biosynthesis;<br>
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L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi<br>
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Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress.<br>
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<b>Aim:</b> We can use the overproduced cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, we need glutamate-cysteine ligase to catalyse the reaction. We can over-express GSH1* using the pYodA promoter. <br>
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<b>Mutant strains in ''E. coli'':</b> Information about mutant strains for ''Gsh1'' from the Keio collection can be found at [http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0413&CFID=9377833&CFTOKEN=d775499c2963924f-23DC4F02-CF19-BFB5-F290A2DBAAB49113&jsessionid=b63022c18b939163d6b5485d476425935481TR EchoBase website] hosted by the University of York
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<b>Literature:</b><ol><li>http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C</li></ol></p></div>
  
 
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Latest revision as of 17:09, 18 October 2014

LacRS + Gsh1*

Lac Repressor System + Gsh1

Original Gene: gsh1/gshA
Organism: Saccharomyces cerevisiae
Protein: Glutamate-Cysteine Ligase (previously known as gamma-glutamylcysteine synthetase)
Function: Gamma glutamylcysteine synthetase catalyzes the first step in glutathione (GSH) biosynthesis;
L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi
Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress.
Aim: We can use the overproduced cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, we need glutamate-cysteine ligase to catalyse the reaction. We can over-express GSH1* using the pYodA promoter.
Mutant strains in E. coli: Information about mutant strains for Gsh1 from the Keio collection can be found at [http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0413&CFID=9377833&CFTOKEN=d775499c2963924f-23DC4F02-CF19-BFB5-F290A2DBAAB49113&jsessionid=b63022c18b939163d6b5485d476425935481TR EchoBase website] hosted by the University of York

Literature:
  1. http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C
</p></div>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1800
  • 1000
    COMPATIBLE WITH RFC[1000]