Difference between revisions of "Part:BBa K1526009:Experience"
 
(18 intermediate revisions by the same user not shown)
Line 6: Line 6:
 
<p>In order to characterize the parts we have created a new BioBrick compatible with our chassis. As  
 
<p>In order to characterize the parts we have created a new BioBrick compatible with our chassis. As  
 
<i>E.coli DH5α</i> does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)</p>
 
<i>E.coli DH5α</i> does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)</p>
−
<img src="https://static.igem.org/mediawiki/2014/6/66/York_Construct.PNG" style="border:1px solid orange">
 
−
<p>Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.</p>
 
  
−
<p>We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into <i>E.coli DH5α</i>
+
<p>We have successfully assembled the parts together by step-wise 3A Assemblies, transformations and gel checking for expected sizes.
 +
 
 +
<p><p>We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into <i>E.coli DH5α</i>
 
</p>
 
</p>
  
−
[[File:Plates_LacRS_I.jpg]]>
+
<p>Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.</p>
 +
[[File:Plate_Resized.jpg]]
 +
<p>Left plate: <i>E.coli DH5α</i> transformed with BBa_K1526009 in PSB1A3 and plated on LB-Agar+AMP+0.5mM IPTG; Right plate: same treatment without IPTG
  
 
===User Reviews===
 
===User Reviews===
Line 20: Line 22:
 
|-
 
|-
 
|width='10%'|
 
|width='10%'|
−
<partinfo>BBa_K1526009 AddReview number</partinfo>
+
<partinfo>BBa_K1526009 3</partinfo>
−
<I>Username</I>
+
<I>Gyaros</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
Working!
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K1526009 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1526009 EndReviews</partinfo>

Latest revision as of 17:05, 18 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Creating the regulator LacRS

In order to characterize the parts we have created a new BioBrick compatible with our chassis. As E.coli DH5α does not have a lac repressor system we have assembled together basic parts J32100, B0033, C0012, B0010, B0012 and R0010 to create the composite regulator Lac Repressor System (LacRS)

We have successfully assembled the parts together by step-wise 3A Assemblies, transformations and gel checking for expected sizes. <p><p>We have successfully cloned and tested the functionality of LacRS in pSB1A3 (<a href="https://parts.igem.org/Part:BBa_K1526009">BBa_K1526009</a>) into E.coli DH5α

Our first functionalization test coupling LacRS upstream the Green Fluorescent Protein (GFP) Biobrick (E0040) showed expression of GFP under UV light when the system is previously induced by IPTG.

Plate Resized.jpg

Left plate: E.coli DH5α transformed with BBa_K1526009 in PSB1A3 and plated on LB-Agar+AMP+0.5mM IPTG; Right plate: same treatment without IPTG

User Reviews

UNIQ9aa4f3b14bf9b3df-partinfo-00000000-QINU

UNIQ9aa4f3b14bf9b3df-partinfo-00000001-QINU