Difference between revisions of "Part:BBa J58101"
 
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<partinfo>BBa_J58101 short</partinfo>
 
<partinfo>BBa_J58101 short</partinfo>
  
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A promoter activated synergistically by cI and CRP proteins (part <partinfo> J58100 Icon</partinfo>) with a generator of the reporter RFP (part ref. BBa_I13507). This generator uses the following parts: BBa_B0034 (RBS), BBa_E1010 (mRFP1), and BBa_B0015 (Terminator). With this construction, we obtain an AND gate, with RFP as output, and cI and CRP as inputs.
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This composed part contains a two-regulator AND promoter activated synergistically by cI and CRP proteins:<partinfo>J58100</partinfo>joined to a generator of the reporter RFP<partinfo>BBa_I13507</partinfo>The deep components are as follows:<partinfo>J58101 DeepComponents</partinfo>With this construction, we obtain a genetic device (not using POPS) that produces RFP when enough levels of cI and CRP are acheived simultaneously.
  
 
'''By Valencia iGEM 2006'''
 
'''By Valencia iGEM 2006'''
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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See comments in <partinfo>J58100</partinfo>
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_J58101 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J58101 SequenceAndFeatures</partinfo>
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See comments in <partinfo>J58100</partinfo>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 21:42, 29 October 2006

Promoter activated synergistically by cI and CRP with the reporter RFP

This composed part contains a two-regulator AND promoter activated synergistically by cI and CRP proteins:BBa_J58100joined to a generator of the reporter RFPBBa_I13507The deep components are as follows:
pAND
J58100
B0034		mRFP1
E1010
B0010
B0012
With this construction, we obtain a genetic device (not using POPS) that produces RFP when enough levels of cI and CRP are acheived simultaneously.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Usage and Biology

See comments in BBa_J58100

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal AgeI site found at 687
    Illegal AgeI site found at 799
  • 1000
    COMPATIBLE WITH RFC[1000]

See comments in BBa_J58100