Difference between revisions of "Part:BBa K1403012"

Line 5: Line 5:
 
<li>Constitutive promoter BBa_J23108</li>
 
<li>Constitutive promoter BBa_J23108</li>
 
<li>RBS BBa_B0030</li>
 
<li>RBS BBa_B0030</li>
<li>CDS: Alcohol acetyltransferase I (ATF1) from <i>Saccharomyces cerevisiae</i></li>
+
<li>CDS: ATF1 from <i>Saccharomyces cerevisiae</i></li>
 
<li>Terminators BBa_B0010 & BBa_B0012</li>
 
<li>Terminators BBa_B0010 & BBa_B0012</li>
 
</ul>
 
</ul>

Revision as of 16:24, 18 October 2014

Alcohol acetyltransferase I (ATF1) expression cassette

  • Constitutive promoter BBa_J23108
  • RBS BBa_B0030
  • CDS: ATF1 from Saccharomyces cerevisiae
  • Terminators BBa_B0010 & BBa_B0012

Transform E. coli to produce isoamyl acetate. Requieres isoamyl alcohol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]