Difference between revisions of "Part:BBa K1403009"
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<ul> | <ul> | ||
− | <li>Constitutive promoter BBa_J23108 | + | <li>Constitutive promoter BBa_J23108</li> |
− | <li>Synthetic RBS | + | <li>Synthetic RBS</li> |
− | <li>CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) | + | <li>CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) from <i>Petunia × hybrida</i></li> |
− | <li>Histidine Tag | + | <li>Histidine Tag</li> |
</ul> | </ul> | ||
Revision as of 16:01, 18 October 2014
Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) from Petunia × hybrida
- Histidine Tag
Transform E. coli and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]