Difference between revisions of "Part:BBa K1403006"
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<ul> | <ul> | ||
− | <li>Constitutive promoter BBa_J23108 | + | <li>Constitutive promoter BBa_J23108</li> |
− | <li>Synthetic RBS | + | <li>Synthetic RBS; TIR: 55464.92</li> |
− | <li>CDS: Jasmonic acid carboxyl methyltransferase (JMT) | + | <li>CDS: Jasmonic acid carboxyl methyltransferase (JMT) from <i>Arabidopsis thaliana</i></li> |
− | <li>Histidine Tag | + | <li>Histidine Tag</li> |
</ul> | </ul> | ||
Revision as of 16:00, 18 October 2014
Jasmonic acid carboxyl methyltransferase (JMT) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS; TIR: 55464.92
- CDS: Jasmonic acid carboxyl methyltransferase (JMT) from Arabidopsis thaliana
- Histidine Tag
Transform E. coli for methyl jasmonate production. Requires jasmonic acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 222
Illegal BamHI site found at 793 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]