Difference between revisions of "Part:BBa K1526006"

Latest revision as of 15:54, 18 October 2014

LacRS + SpPCS

Lac Repressor System + SpPCS

This Biobrick was created in order to allow SpPCS gene characterization in E. coli DH5A
Gene: spPCS
Organism: Schizosaccharomyces pombe
Function: The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group to synthesise phytochelatins.
Aim: Over-expression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.

Literature

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1207
Illegal BamHI site found at 1879
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Lac Repressor System + ''SpPCS''
-<b>This Biobrick was created in order to allow ''SpPCS'' gene characterization in ''E. coli DH5A''
+<b>This Biobrick was created in order to allow ''SpPCS'' gene characterization in '''''E. coli DH5A'''''<br>
-<b>Gene:</b> ''spPCS''<br>
+Gene:</b> ''spPCS''<br>
 <b>Organism:</b> <i>Schizosaccharomyces pombe</i><br>
 <b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group  to synthesise phytochelatins.<br>