Difference between revisions of "Part:BBa K1403019"
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<partinfo>BBa_K1403019 short</partinfo>
 
<partinfo>BBa_K1403019 short</partinfo>
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<ul>
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<li>Constitutive T7 promoter
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<li>RBS
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<li>CDS: Acetohydroxybutanoate synthase/acetolactate synthase (IlvB/N) from <i>E. coli</i> MG1655
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</ul>
  
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ilvBN CDS from E. coli MG1655
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Transform <i>E. coli</i> to overexpress <i>ilvB<i/> and thus increase the basal production of alpha acetolactate, the precursor of acetoin.
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* This was submitted in a pETDuet-1
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ilvBN gene exists in natural wild type E.Coli strain. They codes for acetolactate synthase process. Here, we isolated it from MG1655 strain through PCR to transform it into targeted strain for increased basal production of acetolactate, which serves as precursor for acetoin production.  
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<!-- Add more about the biology of this part here
 
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Revision as of 15:51, 18 October 2014

Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette

  • Constitutive T7 promoter
  • RBS
  • CDS: Acetohydroxybutanoate synthase/acetolactate synthase (IlvB/N) from E. coli MG1655

Transform E. coli to overexpress ilvB<i/> and thus increase the basal production of alpha acetolactate, the precursor of acetoin.

  • This was submitted in a pETDuet-1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]