Difference between revisions of "Part:BBa K1403017"
Line 2: Line 2:
 
<partinfo>BBa_K1403017 short</partinfo>
 
<partinfo>BBa_K1403017 short</partinfo>
  
−
aldB CDS under constitutive promoter from Anderson's promoter library.
+
<ul>
 +
<li>Constitutive promoter BBa_J23108
 +
<li>Synthetic RBS
 +
<li>CDS: &945;-acetolactate decarboxylase (aldB)
 +
<li>Terminators BBa_B0010 & BBa_B0012
 +
</ul>
  
−
It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter. This plasmid was transformed together with Part:BBa_K1403019 to produce butter smell in E.Coli.
+
Transform <i>E. coli</i> to produce acetoin.
 +
The enzyme requires &945;-acetolactate.
 +
A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:36, 18 October 2014

Acetaldehyde dehydrogenase (aldB) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: &945;-acetolactate decarboxylase (aldB)
  • Terminators BBa_B0010 & BBa_B0012

Transform E. coli to produce acetoin. The enzyme requires &945;-acetolactate. A co-transformation with part BBa_K1403019 increases the amount of substrate for aldB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Verification

AldB.png

Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter