Difference between revisions of "Part:BBa K1403009"

 
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__NOTOC__
 
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<partinfo>BBa_K1403009 short</partinfo>
 
<partinfo>BBa_K1403009 short</partinfo>
  
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RBS for tmm
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<ul>
 +
<li>Constitutive promoter BBa_J23108
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<li>Synthetic RBS
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<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)
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<li>His Tag
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</ul>
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Transform E. coli and grow in the prescence of jasmonic acid for methyl jasmonate biosynthesis.
  
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===Usage and Biology===
 
  
 
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Revision as of 14:01, 18 October 2014

Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: Jasmonic acid carboxyl methyltransferase (JMT)
  • His Tag

Transform E. coli and grow in the prescence of jasmonic acid for methyl jasmonate biosynthesis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]